Correlation between AKT activation and expression of the different members of the PI3K pathway in NSCLCs.

aAKT activation was evaluated with as pS473 positivity and scored as negative (<10% of positive tumour cells) and high (>10% of positive tumour cells). bPIK3CA was graded as positive (>25% of tumour cells showed strong or diffuse immunopositivity) as moderate (>10% of tumour cells showed moderate immunopositivity) or negative (0–10% of the tumour cells showed weak, focal immunopositivity or absence of staining). cPTEN expression was classified as (+) when staining was detected in >50% of the cells, (+/−) when staining was detected in 25–50% of cells and (−) when staining was detected in 0–25% of cells. For statistical analysis PTEN expression was considered lost when samples were classified as (−). dAKT1 was graded as positive (>25% of positive tumour cells) as moderate (>10% of of positive tumour cells) or negative (0–10% of positive tumour cells). eAKT2 was graded as positive (>25% of positive tumour cells) as moderate (>10% of positive tumour cells) or negative (0–10% of positive tumour cells). §Statistically significant.