Additional file 2 of Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)

Additional file 2: Supplementary tables 1-7. Table S1. Primers used for vectors construction. Table S2. Primers used for positive test. Table S3. Barcode primers for detecting off-target in base editing T0 transgenic plants with deep sequencing for GhPEBE gene. Table S4. Barcode primers for detecting off-target in base editing T0 transgenic plants with deep sequencing for GhCLA gene. Table S5. Summary of genome-wide potential off-targets predictions by Cas-OFFinder tools for target sgRNA1. Table S6. Summary of genome-wide potential off-targets predictions by Cas-OFFinder tools for target sgRNA2. Table S7. Expression levels (TPM) of TadA, nCas9 and dCas9 in GhABE7.10 edited plant.