genes in primary cultured grass carp pituitary cells regulated by prolactin-releasing peptide

To investigate the direct Prolactin-releasing peptide in grass carp pituitary, Prolactin- releasing peptide (1uM) was used to incubate the primary cultured pituitary cells derived from prepubertal grass carp. After 24h challenge, a high-throughput RNA-seq technique was used to compare mRNA expression profiles between control and prolactin-releasing peptide- treatment group.