Overview of technical performance of the ELISA assays.

The technical performance of assayed used in this study was summarized and listed in this table. The lower limit of detection (LLOD) was computed as 3 Standard Deviation (SD) of the mean value of 21 zero standards. Intra-assay CV% (Intra-assay coefficient of variation, within a plate) and Inter-CV% (inter-assay coefficient of variation, between different plates) were calculated as the mean value of the variation of 8 samples analyzed 10 times in duplicate. Overview of technical performance of the ELISA assays.