Establishment of functional protein interaction networks of so far undescribed human mitochondrial proteins by q-AP-MS

The overall aim of the project is the definition and characterization of the mitochondrial proteome of human cells, referred to as MitoCoP. To this end, we combined complementary strengths of subtractive proteomics, ImportOmics and subcellular protein profiling in a global multidimensional classification approach (see dataset 'Definition and characterization of the mitochondrial proteome of human cells (MitoCoP)'). SILAC-based affinity purification-mass spectrometry (q-AP-MS) experiments were performed to establish functional protein interaction networks of so far undescribed mitochondrial proteins.