Expression data from CRS with nasal polyps (CRSwNP) and normal control

Chronic rhinosinusitis (CRS) is a prevalent chronic rhinologic disease, characterized by persistent symptomatic inflammation of the nasal mucosa and paranasal sinuses. Sinonasal tissues were obtained during sinus surgeries performed at Northwestern University. For the microarray, NP tissues and uncinate tissues from normal healthy controls were divided into elderly (≥65 years old, each n=3-4), or non-elderly (18-49 years old, each n=3-4) according to age. This study protocol was approved by the independent ethics committees or institutional review boards (IRB) at Northwestern University. Total RNA was isolated from NP tissues and control uncinate tissues. RNA concentration was calculated, and the highest quality RNA used for microarray. The quality of total RNA was evaluated by Bioanalyzer (Agilent Technologies, Santa Clara, CA). The samples were at a concentration ≥125ng/μl and a A260/A280 ratio that was ≥1.80 and a RIN value that was ≥7.0 were considered acceptable. The highest quality RNA was used to generate labeled cRNA and hybridized to Affymetrix Human Gene 2.1ST array following the manufacturer’s protocol (Affymetrix Inc., Santa Clara, CA). Chip processing and image capturing were performed using Affymetrix AGCC software. We investigated age- and disease-related differential gene expression in chronic rhinosinusitis with nasal polyps. Microarrays using NP and uncinate tissues from health controls (elderly, age ≥65 vs. non-elderly, age 18-49) were performed, and gene expression profiles were evaluated using Affymetrix Human Gene 2.1ST array.

慢性鼻-鼻窦炎（Chronic rhinosinusitis, CRS）是一种高发的慢性鼻科疾病，以鼻黏膜与鼻窦黏膜持续性症状性炎症为核心特征。研究所用的鼻窦组织样本均取自西北大学开展的鼻窦手术术中获取的标本。本次微阵列实验中，研究人员将健康对照者的鼻息肉（nasal polyps, NP）组织与钩突组织按年龄划分为两组：老年组（年龄≥65岁，每组样本量n=3~4）与非老年组（年龄18~49岁，每组样本量n=3~4）。本研究方案已通过西北大学独立伦理委员会或机构审查委员会（Institutional Review Board, IRB）的伦理审批。从鼻息肉组织及对照钩突组织中分离总RNA，测定RNA浓度，选取质量最优的RNA用于微阵列实验。总RNA的质量通过生物分析仪（Agilent Technologies，美国加利福尼亚州圣克拉拉）进行评估，合格标准为：RNA浓度≥125ng/μl，A260/A280比值≥1.80，RNA完整性数（RIN）≥7.0。选取符合上述质量标准的最优RNA合成标记cRNA，按照制造商（Affymetrix Inc.，美国加利福尼亚州圣克拉拉）的标准操作流程，将其与Affymetrix人类基因2.1ST芯片进行杂交。芯片处理与图像采集工作均采用Affymetrix AGCC软件完成。本研究旨在探究伴鼻息肉的慢性鼻-鼻窦炎中与年龄及疾病相关的差异基因表达特征。实验分别使用老年健康对照（≥65岁）与非老年健康对照（18~49岁）的鼻息肉组织及钩突组织开展微阵列检测，并通过Affymetrix人类基因2.1ST芯片分析基因表达谱。

搜集汇总

背景与挑战

背景概述

该数据集是一个关于慢性鼻窦炎伴鼻息肉（CRSwNP）和正常对照的基因表达数据集，使用Affymetrix Human Gene 2.1ST芯片平台，包含14个样本，按年龄分为老年（≥65岁）和非老年（18-49岁）组，旨在研究年龄和疾病相关的差异基因表达。数据来源于鼻息肉和正常对照组织，通过微阵列实验获取，并已公开供研究使用。