Exploring the interaction mechanisms of extracellular vesicles with model membranes

Owing to the content of the Extracellular Vesicles (EVs), they can target their cargoes to control and mediate the intercellular communications by both paracrine and endocrine mechanism. Yet, their exact interaction mechanism is not yet fully elucidated. Therefore, we address this by a multi-technique approach, using model membranes of different compositions. We isolated EVs from murine Neuro2a (N2a) neuroblastoma cells, treated and untreated with the oligosaccharide OligoGM1, known for its neurotrophic and protective functions in Parkinson’s disease. Those 2 types of EVs were characterized by Western Blotting, DLS, NTA, AFM, DSC, FT-IR and NR, and have already shown different chemico-physical characteristics. However, we believe that SAXS and WAXS experiments will help to disclose more precise structural information of their detailed and different interaction modes with target membranes helping to understand how the treatment with OligoGM1 affects the intercellular communication.