GENSCAN translation prediction scores for expression of wild-type (wt) and codon-optimized (co) lux genes in a mammalian host cell (http://genes.mit.edu). Predicted start position is the predicted nuc
Restriction/ligations were performed from 5 to 30 minutes, and either immediately transformed in E.coli (Digest an heat inactivation/no) or incubated 5 minutes at 50°C and 5 minutes at 80°C (Digest an
