A case-control study to compare the placental microbiome of preeclampsia and normotensive pregnant women. Microbiota in preeclampsia placenta

By 16S rRNA sequencing, placenta microbiota were compared between 24 preeclampsia and 22 normotensive pregnant women. During experimental processing, we monitored contamination by adding DNA-free water during extraction and amplification, as well as a sterile swab during placental sampling. The result showed that placental metagenomes were significantly distinct in their clustering from negative controls. Classification from contaminant filtered data identified a distinct taxonomic makeup between the preeclampsia and normotensive placentas