3D-Bioprinting allows the establishment of long-term 3D culture model for Chronic Lymphocytic Leukemia cells

Studying primary Chronic Lymphocytic Leukemia cells in vitro is very challenging due to their short survival in culture, and also due to the fact that traditional two-dimensional in vitro models lack cellular and spatial complexity present in vivo. Based on these considerations, we exploited three-dimensional (3D) bioprinting to advance in vitro models for CLL. Through RNA sequencing (RNAseq) analysis, we observed a consistent difference in gene expression profile between 2D and 3D samples, indicating a different behavior of the cells in the two different culture settings. Examination of the transcriptomic differences between traditional two-dimensional (2D) cultured cells and cells cultured in three-dimensional (3D) bioprinted scaffolds. RNA from bioprinted 3D cells was extracted overtime, after 7 and 14 days.