Examining the role of ATRX-RNA interactions in ATRX and PRC2 localization and gene expression.

We disrupted the RNA binding region in endogenous ATRX and tested localization of ATRX, EZH2 and H3K27me3 by CUT&RUN. We show that ATRX-RNA binding is important to regulate ATRX and PRC2 localization to specific genes. Overall design: We performed CUT&RUN for ATRX, EZH2 and H3K27me3 in WT and ATRX?RBR (ATRX with RNA binding region deleted) using the protocol described in Skene et al, Nature protocols, 2018). RNA Seq was performed in WT and ATRX?RBR.