Single-cell Transcriptome Landscape and Cell Fate Decoding in Human Brain Organoids after Transplantation

Human stem cells and derivatives have been widely used to treat neurological diseases, and the fate of transplantation cells has not been well clarified. In order to explore the changes in fate of the human brain organs (HBOs) before and after transplantation, we transplanted HBOs into the prefrontal cortex (PFC) and hippocampus (HIP), respectively. Then we performed single-cell transcriptome sequencing. We obtained a total of 5 samples: before transplantation (group D0) and four groups of samples after transplantation (group HIP-2MPT, PFC-2MPT,HIP-4MPT,and PFC-4MPT). Our studies have shown that after transplantation, HBO has accepted the regulation of host's factors, which leads to the similarity of HBOS cell fate to the brain area of transplantation. It may help better understand cell transplantation and regeneration medicine.