Generation of functional hepatocytes from extended pluripotent stem cells

We established efficiently differentiation protocol of human extented pluripotent stem (EPS) cells to functional hepatocytes (EPS-Heps) by simply adding a pre-treatment step before applying the directed differentiation protocol for conventional human pluripotent stem cells. Importantly, compared to hepatocytes derived from conventional human pluripotent stem cells, EPS-Heps transcriptionally more resemble primary human hepatocytes. Examination of mRNA expression pattern of EPS cells, primed pluripotent stem cells, differentiated cells and freshly isolated fetal liver and freshly isolated primary hepatocytes (F-PHHs).