Stacking potato NLR genes (PRJCA036635)

Here, we generated transgenic 2R and 3R potato lines by stacking R genes Rpi-blb2/Rpi-vnt1.1 and Rpi-vnt1.1/RB/R8, respectively, in the susceptible cv. Desiree background. We performed transcriptome analysis of transgenic potato lines that response to P. infestans in transgenic lines carrying single R genes vs lines carrying stacks of multiple R genes.