The South African national combination antiretroviral therapy (cART) roll-out program started in 2006, with over 4.4 million people accessing treatment since it was first introduced. HIV-1 drug resist
*F indicates forward primer orientation, R indicates reverse primer orientation. †Primer used for both PCR and sequencing reactions (26) ‡Sequencing primer [27] §Sequencing primer (designed by Dr. Gle
α,β-D-CNA TT is indicated by T*T and triethylene glycol phosphate unit by Peg in the primer sequences. The modification of lengths due to the insertion of α,β-D-CNA TT or triethylene glycol phosphate