MiRNA (serum) validation of AIONFH cases by Real-time PCR

To further validate the array results, qRT-PCR was performed. Our results showed that miR-127, miR-628-3p, miR-1, and miR-432-5p were downregulated (P<0.05), miR-885-5p, miR-483-3p, and miR-483-5p were upregulated (P<0.05), and miR-654-3p, miR-323a-3p, and miR-582-3p were unchanged. Collectively, these qRT-PCR results confirmed seven differentially expressed miRNAs using microRNA PCR Panels; however, three miRNAs were not significantly different based on qRT-PCR.