Differential Scanning Fluorimetry of Nsp3 macrodomain of SARS-CoV-2

This folder contains DSF data presented for the manuscript "Fragment Binding to the Nsp3 Macrodomain of SARS-CoV-2 Identified Through Crystallographic Screening and Computational Docking" published in Science Advances in 2021. It contains the raw data, in both normalized and non-normalized form, as well as the apparent melting temperatures. For convenience, each dataset is included in at least two different formats, but the data is the same between the differently-formatted files. The raw data, as seen in Supplementary Figure 9, and Supplementary Dataset 2 (Files 1-4 contain identical data, in different formats, for user convenience) File 1 - To read into R as "long form" data, both normalized and non-normalized RFU: 2021_sci_advances_nsp3_mac1_dsf_raw_data_long_form.rds File 2 - A csv, identical in structure to File 1: 2021_sci_advances_nsp3_mac1_dsf_raw_data_long_form.csv File 3 - A csv containing "wide-form" RFU data: 2021_sci_advances_nsp3_mac1_dsf_raw_rfu_data_wide_form.csv File 4 - A csv containing "wide-form" normalized RFU data: 2021_sci_advances_nsp3_mac1_dsf_raw_rfu_data_normalized_wide_form.csv The Tma data, as seen in Supplementary Figure 9, Supplementary Dataset 1, and Supplementary Dataset 2: (Files 5-6 contain identical data, in different formats, for user convenience) File 5 - To read into R as "long form" data: 2021_sci_advances_nsp3_mac1_dsf_tmas.rds File 6 - A csv, identical in structure to File 5: 2021_sci_advances_nsp3_mac1_dsf_tmas.csv