Small RNA-seq of human HEK293 cells overexpressing mmu-miR-1948 primary sequence

Ectopic expression of mmu-miR-1948 primary sequence in HEK293 cells was followed by detection of mature mmu-miR-1948 by small RNA-seq to verify the authenticity of miR-1948 The mmu-miR-1948 hairpin sequence plus the surrounding ~200 bp sequence were overexpressed in HEK293 cells by adenovirus infection. Small RNA-seq was performed on the infected HEK293 cells to determine the presence of processed miR-1948 mature microRNAs.