Ptms unlocks chromatin, Fig 7

Brain glial progenitor cells in culture spontaneously transform into neoplastic cells with tumorigenic potential. Transcript analysis identified a histone binding protein up regulated during this process. An examination of its function when introduced into normal cells revealed a dramatic affects on chromatin, with decreased DNA methylation and induction of glioma identity genes. These files contain individual fasta genomic sequences of rat brain glial cells (PC, files djt69), and human embryo kidney (HEK, files djt70, djt86) transfected with a plasmid expression vectors then treated with Ct conversion reagent (Zymo Research, Cat #D5001-1) to distinguish methylated and non-methyl CpG sites. 69-1 OPC x eGFP 69-2 OPC x Ptms 69-3 OPC x H1a 69-4 OPC x H1a,Ptms 70-2 HEK x Ptms 70-3 HEK x H1a 70-4 HEK x H1a Ptms 86-1 HEK x eGFP 86-2 HEK x Ptms 86-3 HEK x H1a 86-4 HEK x H1a, Ptms