[KIL-d] experiments. [KIL-d] experiments

In yeast, the extrachromosomal genetic element [KIL-d] alters killer activity of M double–stranded RNA killer virus and confers cell resistance against the killer virus. However, its underlying mechanism and the molecular nature of [KIL-d] is unknown. We found [KIL-d] selectively increases the rate of de novo mutation in the killer toxin gene of the viral genome. To verify this hypotesis, we performed deep sequence analysis to calculate mutation rate.