Single-Cell Analysis of Age-Related Changes in Leukocytes of Diabetic Mouse Hindpaws. Single-Cell Analysis of Age-Related Changes in Leukocytes of Diabetic Mouse Hindpaws

We used single cell RNA sequencing to identify transcriptomic changes in immune cells of the distal limb in diabetic Leprdb/db mice at two different ages that have the potential to contribute to the complications of the distal limb associated with diabetes Overall design: Single cell RNA sequencing to identify transcriptomic changes in immune cells of the distal limb in diabetic Leprdb/db mice at two different ages. Cells were isolated from mice hindpaws and performed fluorescence-activated cell sorting (FACS) to separate the immune cells and then performed scRNAseq