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DNA methylation analysis of HCT116 after knockdown and rescue of UHRF1. DNA methylation analysis of HCT116 after knockdown and rescue of UHRF1

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NIAID Data Ecosystem2026-03-11 收录
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In HCT116 colorectal cancer cells, UHRF1 was knocked down by shRNA (puromycin) while simultaneously transduced with wildtype or mutant UHRF1 (blasticidin) or NDI1 (- control) followed by dual antibiotic selection. DNA was analyzed 11 days after viral transduction. Overall design: Bisulphite converted DNA from the 4 samples were hybridised to the Illumina Infinium MethylationEPIC BeadChip.

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2019-08-13
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