RNA sequencing of miRNA in plasma from myocardial ischemia and reperfusion mice and sham mice

Purpose: The aim of this study is to compare the plasma miRNA profile between mice subject to myocardial ischemia and reperfusion and mice subject to sham operation. Methods: 8 to 10-week old C57BL/6 mice underwent myocardial ischemia and reperfusion (MIR) or Sham operation. Plasma RNA was isolated using Trizol LS reagent 4h post-surgery. NGS cDNA libraries were prepared using Norgen Biotek Small RNA Library Prep Kit. Library quality was validated prior to sequencing on an Illumina NextSeq 500 platform. 8 to 10 weeks old male C57BL/6 mice were subject to myocardial ischemia and reperfusion (8 mice) or sham surgery (6 mice). Plasma was collect for RNA isolation 4 hour post-surgery. cDNA librarues were sequenced using Illumina NextSeq 500 platform.