Generation of a conditional knockout mouse of the <i>Cc2d1b</i> gene locus.

(A) Scheme for generating animals carrying the conditional knockout allele (Cc2d1bflox) and the recombined allele (Cc2d1b-). LoxP sites are depicted as yellow triangles, FRT sites as orange triangles. The DM14 and C2 protein domains are marked in green and red, respectively. Primers for genotyping are depicted as black arrows. (B) PCR genotyping of wild-type and heterozygous and homozygous Cc2d1b- mice using primers 1F, 3R and 3bR. (C) RT-PCR analysis of total RNA extracts of brain of newborn animals with exon specific primer pairs that confirm lack of expression of the deleted segment. (D) Immunoblotting of protein lysates from MEF (mouse embryonic fibroblast) cells isolated from Cc2d1b-/- and control mice. The 130kDa CC2D1B band is lacking in the lysates of homozygous Cc2d1b-/- mice.