Additional file 4 of Comparison between 5 extractions methods in either plasma or serum to determine the optimal extraction and matrix combination for human metabolomics

Additional file 4: Figure S4. Heatmaps reflecting the peak area of standard unlabeled analytes from plasmaand serumHeatmap, reflecting the response of the detected unlabeled standards from plasmavs. serumunder five extraction methodswithin four LC-MS analysis. Colour coding: signal responseincreases from light to dark green ; White box : unidentified standard MeOH : methanol; MeOH:ACN : methanol combined with acetonitrile; ACN: acetonitrile; SPE : solid preparation extraction; ESI : electrospray ionisation; RP: reverse phase.