Lamins organize the global three-dimensional genome from the nuclear periphery

To visualize if HiLands-B LADs indeed move away from the NL upon lamin loss, we selected two regions consisting of mostly HiLands-B and exhibiting decreased emerin DamID values upon lamin loss for FISH analyses using Oligopaint. We used FISH to label the HiLands-B LADs while immunostained on Emerin to mark the nuclear periphery. Using Imaris, we quantified the distance between the FISH signal and the NL and found that the two HiLands-B regions in TKO mESCs were farther away from the NL than in the WT.