Vd1pos NKp46pos IELs and Vd1pos NKp46neg IELs were FACS isolated to perform an RNA-based high-throughput analysis of T-cell receptor repertoires. For this, PCR amplicons of the CDR3 regions of the g-c
This study aimed to test the feasibility of rapid amplification of cDNA ends (RACE) via polyadenylating cDNA and amplifying with a poly-T and a constant region primer
we performed immune repertoire sequencing on five biopsy sites of each tumor and on matched adjacent normal tissues and peripheral blood from five patients diagnosed with PLC, to investigated the spat