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CRISPR off-target enrichment experiments
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创建时间：
2020-05-19
相关数据集
Additional file 10 of Amplification-free long-read sequencing reveals unforeseen CRISPR-Cas9 off-target activity
Additional file 10: Supplementary Data 9. SMRT-OTS results for ATXN10, MMP14 and NEK1 when using de novo assembled HEK293 genome for alignment.
NIAID Data Ecosystem40
DataSheet_1_Efficacy and safety of universal (TCRKO) ARI-0001 CAR-T cells for the treatment of B-cell lymphoma.pdf
Autologous T cells expressing the Chimeric Antigen Receptor (CAR) have been approved as advanced therapy medicinal products (ATMPs) against several hematological malignancies. However, the generation
NIAID Data Ecosystem30
WAS gRNA off-target seqencing
GUIDE-seq whole genome sequencing of HEK293T cells electroporated with a WAS gRNA and HiFi Cas9 ribonucleoprotein complex
NIAID Data Ecosystem20
Optimizing genome editing strategy by primer-extension-mediated sequencing
High specificity of e\ngineered nucleases ensures precise genome editing. Couple methods were developed to identify off-target sites of CRISPR/Cas9, but hardly any high-throughput sequencing method ca
NIAID Data Ecosystem40
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
We describe a robust linear amplification-mediated high-throughput genome-wide translocation sequencing (HTGTS) method that identifies endogenous or ectopic "prey" DNA double-stranded breaks (DSBs) ac
NIAID Data Ecosystem30
