B. subtilis yxjA RIboswitch: Broken Central Helix Mutants and Terminator Controls

Measuring fluorescence of yxjA riboswitch-SFGFP reporter construct in E. coli. Samples were grown in media with or without 2-aminopurine ligand to observe changes in fluorescence in response to riboswitch regulation. Conclusion: The yxjA riboswitch central helix needs to base pair to perform strand exchange and control gene expression in response to ligand binding. 3 replicates tested for each construct/condition, bulk fluorescence data collected for each sample using a plate reader, calibrated relative fluorescence units into MFEL units using 8-peak Rainbow particle calibration beads, QC beads run on flow cytometer prior to performing experiment.