We report here a PCR-based cloning methodology that requires no post-PCR modifications such as restriction digestion and phosphorylation of the amplified DNA. The advantage of the present method is th
MitoSRAI cDNA was PCR and restriction digest cloned from mitoSRAI_pcDNA3 (Provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan; Cat# RDB18223) into pLVX-EF1α-IRES-Puro
