Bioassay-guided detection of antimicrobial components from <i>Sutherlandia frutescens </i>and <i>Salvia africana-lutea</i>

Ethics reference number: CPUT/HW-REC 2020/H12.This study sought to investigate the antibacterial activity of <i>Salvia africana-lutea</i><i> </i>extracts against common skin pathogens such as <i>Staphylococcus aureus, </i>Methicillin-resistant <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i> and <i>Pseudomonas aeruginosa. </i>Phytochemical techniques, such as Thin-Layer Chromatography, Bioautography, Nuclear Magnetic Resonance Spectrometry, and Liquid-Chromatography Mass Spectrometry were employed to detect and possibly identify the active fractions/compounds.<br>伦理审查编号：CPUT/HW-REC 2020/H12。本研究旨在探究黄花鼠尾草（Salvia africana-lutea）提取物对常见皮肤致病菌的抗菌活性，受试致病菌包括金黄色葡萄球菌（Staphylococcus aureus）、耐甲氧西林金黄色葡萄球菌（Methicillin-resistant Staphylococcus aureus）、表皮葡萄球菌（Staphylococcus epidermidis）以及铜绿假单胞菌（Pseudomonas aeruginosa）。本研究采用薄层色谱（Thin-Layer Chromatography）、生物自显影（Bioautography）、核磁共振波谱法（Nuclear Magnetic Resonance Spectrometry）及液相色谱-质谱联用法（Liquid-Chromatography Mass Spectrometry）等植物化学分析技术，对活性组分与化合物进行检测并可能予以鉴定。