Effect of HCQ treatment on intestinal microbiota in IBD mice

Based on protocol of the manufacturer, we performed sequencing of samples on the platform Illumina NovaSeq, which was derived from LC-Bio. In analyzing species diversity complexness, we applied alpha diversity via five indicants, namely Observed species, Goods coverage, Chao1, Simpson and Shannon. The indicants employed in this work were computed via QIIME2. In particular, calculation of beta diversity was accomplished with QIIME2, while plotting of data was performed with R-package. We calibrated the sequence with Blast, wherein SILVA database was used to annotate each illustrative sequence. Implementation of other diagrams was done with R-package (V3.5.2).