Blood DNA from up to 136 ARID patients were enriched in the targeted exons and deep sequenced. The aim is to unravel the variants in the specific linkage intervals and identify the causal mutations.
Multiplex identifiers (MIDs) are underlined. Forward primers use MID7 and reverse primers use MID4 (Roche). Additional complementarity between RT-PCR primers and 454-Adapter PCR primers is identified