Xrn1 knock out A549 cells were infected with WT PR8 or PR8-PA(∆X), or mock infected. 5’ RACE was then performed using primers specific for BCAP31, TUBA1B, INSIG1 or SLC7A5, positioned ~ 200-300 nu
HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase repor
