Supplemental Figure 2: RNAseq-based genotyping of all chromosomes.

Supplemental Figure 2: RNAseq-based genotyping of all chromosomes. Shown are the S. pennellii introgression regions for ILs covering chromosomes 1 to 12 (A-L) as determined by RNA-Seq. The depth of coverage (distance from midpoint on y-axis) and genotype (color and direction on y-axis) of each SNP/indel is plotted against chromosomal position (x-axis). Polymorphisms that match S. pennellii are colored green and plotted on the top half of each IL panel, while polymorphisms matching cv. M82 are plotted in magenta in the bottom halves. The coloring is on a continuum such that the color approaches black as a position’s genotype approaches heterozygosity. The y-axis tick marks indicate depths of coverage ranging from 0 to 100. Subsequent to genotyping, introgression boundaries consistent between the RNA-Seq and RESCAN analyses were delineated. Using these breakpoints, S. pennellii and cv. M82 regions are summarized by horizontal lines at the top and bottom of each IL panel, respectively.