consensus sequences drafted from microbial communities that were characterized with ONT 16S rRNA gene sequencing

Consensus sequences generated by CONCOMPRA, NGSpeciesID, and amplicon_sorter from (1) a synthetic bacterial community composed of 20 equally abundant bacteria (16S MOCK; https://www.atcc.org/products/msa-1002) or (2) the ZymoGut Microbiome Standard, a microbial community mimicking the human gut microbiome (Gut Microbiome Standard; https://files.zymoresearch.com/datasheets/ds1712_zymobiomics_gut_microbiome_standard_data_sheet.pdf). The first was sequenced twice, on both a R9 and and R10 flow cell (indicated by the folder structure). For CONCOMPRA, all the consensus sequences ('CONCOMPRA.all_consensus') are given as well as the non chimeric sequences only ('CONCOMPRA.noglobal_nolocalchim.consensus.sequences'). The full-length of the 16S rRNA gene was amplified with the primer set 27F and 1492R.Sequencing details as well as the data processing steps are described in: Stock, Willem, et al. "Breaking free from references: a consensus-based approach for community profiling with long amplicon nanopore data." <i>bioRxiv</i> (2024): 2024-07. ( https://www.biorxiv.org/content/10.1101/2024.07.04.602031v1 )<br>

本数据集包含由CONCOMPRA、NGSpeciesID及amplicon_sorter生成的共有序列（consensus sequences），其来源分为两类：(1) 由20种丰度均等的细菌构成的合成细菌群落（16S MOCK；https://www.atcc.org/products/msa-1002）；(2) Zymo肠道微生物组标准品（ZymoGut Microbiome Standard），一款模拟人类肠道微生物组的微生物群落标准品（肠道微生物组标准品；https://files.zymoresearch.com/datasheets/ds1712_zymobiomics_gut_microbiome_standard_data_sheet.pdf）。该合成细菌群落（即第一类样本）进行了两次测序，分别采用R9与R10型测序流动池（flow cell），样本分组情况由文件夹结构标注。针对CONCOMPRA工具，本数据集同时提供其全部共有序列（文件标识为'CONCOMPRA.all_consensus'）与仅保留非嵌合序列的子集（文件标识为'CONCOMPRA.noglobal_nolocalchim.consensus.sequences'）。16S rRNA基因的全长序列通过引物对27F与1492R扩增得到。测序细节与数据处理流程详见以下文献：Stock, Willem 等. "Breaking free from references: a consensus-based approach for community profiling with long amplicon nanopore data." *bioRxiv* (2024): 2024-07. (https://www.biorxiv.org/content/10.1101/2024.07.04.602031v1 )