<b>jGCaMP8 transgenic mice</b>

The GENIE Project Team at HHMI Janelia Research Campus have developed and characterized multiple transgenic mice expressing jGCaMP8s and jGCaMP8m and deposited the lines at The Jackson Laboratory (JAX). jGCaMP8s and jGCaMP8m are genetically encoded calcium indicators with fast kinetics (~5 fold shorter rise time) than jGCaMP7f and high sensitivity (2X higher 1AP SNR) than jGCaMP7s (see https://doi.org/10.1038/s41586-023-05828-9 for details). This white paper provides updates on developments in each transgenic mouse line and how to obtain them.The novel mouse lines include:<b>1) Tetracycline‐controlled transactivator protein (tTA)-dependent expression</b>JAX 037717 TetO‐jGCaMP8s<b>2)</b><b> </b><b>Cre recombinase-dependent expression</b>JAX 037718 TIGRE2‐jGCaMP8m‐IRES‐tTA2‐WPREJAX 037719 TIGRE2‐jGCaMP8s‐IRES‐tTA2JAX 037952 TIGRE2-jGCaMP8s-IRES-tTA2-WPREJAX 039267 TIGRE2‐RiboL1-jGCaMP8s‐IRES‐tTA2Browsable 3D histology volumes can be found here.<br>

霍华德·休斯医学研究所（HHMI）贾内利亚研究园区的GENIE项目团队开发并表征了多种表达jGCaMP8s与jGCaMP8m的转基因小鼠品系，并将这些品系存档于杰克逊实验室（The Jackson Laboratory，简称JAX）。jGCaMP8s与jGCaMP8m均为基因编码钙指示剂，相较于jGCaMP7f，其具备更快的动力学特性（上升时间缩短约5倍），且相较于jGCaMP7s，灵敏度提升2倍（单动作电位信噪比更高），详细信息可参见https://doi.org/10.1038/s41586-023-05828-9。本白皮书将介绍各转基因小鼠品系的研发进展，并说明其获取方式。新型小鼠品系包括：
1. 四环素调控反式激活蛋白（tTA）依赖型表达品系：JAX 037717 TetO-jGCaMP8s
2. 重组酶Cre依赖型表达品系：
JAX 037718 TIGRE2-jGCaMP8m-IRES-tTA2-WPRE
JAX 037719 TIGRE2-jGCaMP8s-IRES-tTA2
JAX 037952 TIGRE2-jGCaMP8s-IRES-tTA2-WPRE
JAX 039267 TIGRE2-RiboL1-jGCaMP8s-IRES-tTA2
可浏览的三维组织学数据集可于此处获取。