The transcriptome expression levels related to ovulation induction and acupuncture protection therapy in rats through gene microarray

Figure 1 were the results of HE staining images, and showed that acupuncture enhanced the endometrial receptivity of ovulation induction rats. Figure 2-Figure 6 were the results of gene microarray analyses, and the raw data of gene microarray are available in NCBI GEO database at https://www.ncbi.nlm.nih.gov/geo/info/linking.html, reference number GSE190472. Through analysis, it was found that 446 differential expressed genes (DEGs) and 404 DEGs were respectively screened between model and control groups, and treatment and control groups. After comparison, 189 overlapped DEGs were obtained. Thereafter, these overlapped DEGs were divided into four clusters by Mfuzz algorithm. Afterwards, interaction networks containing 842 interaction pairs were established, and the DEGs in the interaction networks were significantly enriched in 20 BP and 10 KEGG pathways, including wound healing, response to glucocorticoid, TNF signaling pathway, complement and coagulation cascades, terpenoid backbone biosynthesis, and glutathione metabolism. By calculating topological parameters, Anxa5, Casp8, Tgm2, Frk, Mmp7, Timp1, Hmgcr, Cth, Serpinal and Abcbla were the important hub genes of the interaction networks.