Genetic mapping of barley genes.

1Forward (F) and reverse (R) primer; primers highlighted in bold were used for direct sequencing for detection of polymorphisms. 2A/GC = anealing temperature/GC buffer (+ = GC buffer added, − = no GC buffer added). 3GenBank accessions for genomic sequences from OWB-D (D) and OWB-R (R) parental lines are indicated. SNP positions are relative to OWB-D. 4Barley chromosome arm: short (s), long (l). HvCMF5 and HvCMF6b were genetically mapped using presence/absence PCR/agarose gel InDel assays. 5Due to lack of polymorphism in the genomic fragment investigated, HvCO15 was mapped using a SNP in an adjacent predicted gene (HvOs08g42430) located on the same sequence contig (Table S4).