Kinetics of 5′-end activation catalyzed by BtTLP variants.
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aSubstrate is full-length S. cerevisiae tRNAHis (missing G−1 residue) used for standard G−1 addition assays [30]. b Substrate is D. discoideum mitochondrially-encoded tRNAIleCAU missing G+1 residue (transcript initiates with C+2) used for standard tRNA repair assays [13]. c Estimate for kobs values derived from observed rates determined using the method of linear initial rates.
创建时间：
2015-12-02
