Role for citron kinase in prostate cancer growth

To study the effect of siRNA-mediated CIT silencing on androgen-responsive genes in LNCaP cells. Human LNCaP prostate cancer cells were transfected with siRNA SmartPools targeting CIT or a non-targeting siRNA SmartPool. Forty-two hours after transfection, cells were treated with synthetic androgen R1881 (0.1nM) or vehicle. Three biological replicates were generated per treatment group. Forty-eight hours later, total RNA was isolated and processed for RNA-Seq analysis.