Equations and the ANOVA results for the studied responses.

aA, NH4Cl (mM); B, Fructose (mM); C, Pyruvate (mM); D, L-Aspartic acid (mM); E, Arginine (mM); F, MgSO4 (mM); G CaCl2 (mM); H, K2HPO4 (mM); I, HEPES (mM); J, EDTA-Fe3+ (mM); K, FeSO4 (mM); L, CuSO4 (mM); M, ZnSO4 (mM); N, TES (ml); O, Inoculation size (×106 cells/ml); P, pH control (NaHCO3 (−1), NH4HCO3 (+1)); Q, Agitation speed (rpm); R, Temperature (°C); S, Time (h).bCN, NH4Cl concentration (mM);TES, trace element solution (ml);AS, Agitation speed (rpm); T, Time (h).cR2, coefficient of determination.dAdj.R2, Adjusted R2.ePred.R2, Predicted R2.