RNA sequencing of tomato seed tissues matured in different environments. RNA sequencing of tomato seed tissues matured in different environments

During maturation seeds acquire several physiological traits to enable them to survive drying and disseminate the species. The maternal environment during maturation can influence seed quality. RNA-seq analyses of the embryo and the endosperm of seed matured ex planta under standard or stressful condition (high temperature and/or dim light) will underly how light and temperature and a combination of both have an impact on the molecular mechanisms governing seed quality at harvest. Overall design: Fruits at breaker stage (i.e 63 DAF) were collected from mother plants grown in greenhouse and transfered to growth chambers under 4 different environments: standard temperature + high light; standard temperature + dim light; high temperature + high light or high temperature + dim light. Three or four replicates of 10 fresh tomato seeds were harvested at mature stage for each maturation environment. RNA-seq data of isolated seed tissues was obtained for each maturation condition