Transcriptomic changes in FOXP3 deficient CD8+ T cells. Transcriptomic changes in FOXP3 deficient CD8+ T cells

Characterization of the functional differences observed by the absence of FOXP3 in CD8+ T lymphocytes. Highly purified OT-1 Foxp3Scurfy and OT-1 Foxp3wt T cells from the corresponding BM chimeric mice were isolated and left unstimulated or stimulated with SIINFEKL peptide for 6h in culture medium. RNA was then isolated and sequenced for the analysis of differential expression of mRNA transcripts Overall design: OT-1 Foxp3Scurfy and OT-1 Foxp3wt T cells were left unstimulated or stimulated with SIINFEKL peptide for 6h. mRNA was then purified and analyzed by RNA-sec