Omicron变异株攻毒后肺部病毒核酸载量
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该数据集来自于本项目筛选的两种对不同的新冠病毒抗原具有高亲和力和高中和活性的中和抗体（F61和H121）的抗体中和效果研究，其中包含小鼠肺病毒核酸载量。当针对新冠病毒Omicron变异株进行测试时，F61和H121的中和活性保持不变，表明研发的两种抗体对SARS-CoV-2变异具有广泛的中和活性。对于肺病毒核酸载量检测，使用RNA/DNA纯化试剂盒（磁珠）（货号DA0623；中国达安基因有限公司）从肺组织匀浆中提取总RNA，并按照制造商的说明使用2019-nCoV PCR检测试剂盒（荧光法）（Fast）（货号DA0992；达安基因股份有限公司）进行qRT-PCR，每个样本设置两个复孔，使用以下程序进行荧光定量PCR检测：50℃ 2 min, 95℃ 2 min, 接下来进行42次循环：95℃ 5 s，60℃ 10 s。用SARS-CoV-2的RNA标准品建立标准曲线，测定小鼠肺部的病毒RNA浓度（copies/mL）（广州邦德胜生物技术有限公司，中国广州）。根据达安新冠核酸检测试剂盒标准读取qRT-PCR结果，数据描述中的阴性结果意味着未检测到信号（0 copies/mL），或者N和ORF1ab基因的CT值均超过38，相应的病毒RNA拷贝数低于检测限（LOD，102.7copies/mL）。所述数据通过Applied Biosystems实时荧光定量 PCR 仪器上采集获得，采集时间为2022年1月，采集地点为中国疾病预防控制中心病毒病预防控制所。
This dataset is derived from a study evaluating the neutralization efficacy of two neutralizing antibodies (F61 and H121) screened in this project, which exhibit high affinity and potent neutralizing activity against various SARS-CoV-2 antigens, and it contains viral nucleic acid load data in mouse lungs. When tested against the SARS-CoV-2 Omicron variant, the neutralizing activities of F61 and H121 remained unchanged, demonstrating that the two developed antibodies have broad neutralizing activity against SARS-CoV-2 variants. For the detection of pulmonary viral nucleic acid load, total RNA was extracted from lung tissue homogenates using an RNA/DNA purification kit (magnetic bead-based, Cat. No. DA0623; Daan Gene Co., Ltd., China), and qRT-PCR was performed following the manufacturer’s instructions using the 2019-nCoV PCR Detection Kit (Fluorescence Method, Fast) (Cat. No. DA0992; Daan Gene Co., Ltd.). Two replicate wells were set up for each sample, and the fluorescence quantitative PCR detection was conducted with the following protocol: 50℃ for 2 min, 95℃ for 2 min, followed by 42 cycles of 95℃ for 5 s and 60℃ for 10 s. A standard curve was established using SARS-CoV-2 RNA standards to quantify the viral RNA concentration in mouse lungs (copies/mL) (Guangzhou Bangdesheng Biotechnology Co., Ltd., Guangzhou, China). qRT-PCR results were interpreted in accordance with the specifications of the Daan COVID-19 nucleic acid detection kit. A negative result in this dataset means no signal was detected (0 copies/mL), or the CT values of both the N and ORF1ab genes exceeded 38, with the corresponding viral RNA copy number below the limit of detection (LOD, 102.7 copies/mL). The data were collected on an Applied Biosystems real-time fluorescence quantitative PCR instrument in January 2022 at the National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
