File S1 - Dihydroflavonol 4-Reductase Genes Encode Enzymes with Contrasting Substrate Specificity and Show Divergent Gene Expression Profiles in Fragaria Species

File includes Figures S1–S3 and Table S1. Figure S1: Left: Ripe strawberry fruits of F. vesca cv. Red Wonder. Right: F.×ananassa cv. Elsanta. Due to differing magnification factors used, fruit size does not appear at a comparable scale. Figure S2: Radioscan of TLC on cellulose from incubation of recombinant DFR2 (left) and DFR1 (right) in the presence of NADPH offering A: (14C)dihydroquercetin, B: (14C)dihydrokaempferol, C and D: (14C)dihydroquercetin and (14C)dihydrokaempferol in equimolar amounts as substrates. Figure S3: Quantitative expression of DFR1 and DFR2 normalized to actin in receptacle and achenes of Fragaria fruits along the different stages of the fruit development. a: F.×ananassa receptacle, b: F.×ananassa achenes, c: F. vesca receptacle, d: F. vesca achenes. red: DFR1, grey: DFR2. Data were calculated from three biological replicates with at least two technical replicates for each and error bars representing the standard deviation. Table S1: List of primers used for quantitative Real-time PCR. (DOC)