Mechanistic dissection of BLTP2 targeting to ER-PM contact sites

The bridge-like lipid transfer proteins (BLTPs) are a novel superfamily of rod-shaped lipid transporters that engage in bulk non-vesicular movement of lipids at organelle membrane contact sites. The molecular and cellular functions of these proteins are still emerging; however, it is clear that one key aspect that regulates BLTP function is targeting to the appropriate membrane contact site(s). Here, we use Drosophila as a model system to dissect the mechanisms that drive targeting of BLTP2 (hobbit in Drosophila) to endoplasmic reticulum-plasma membrane (ER-PM) contact sites. We demonstrate that a conserved adapter protein, which we name bilbobaggins (bbo), is required for targeting of Hobbit to ER-PM contacts; importantly, loss of bbo phenocopies loss of hobbit, indicating that bbo is required for hobbit function. Additionally, our structure-function analyses show that cis-acting sequences in the C-terminal tail of Hobbit are also required for ER-PM targeting. Crucially, our data indic..., , # Data from: Mechanistic dissection of BLTP2 targeting to ER-PM contact sites Dataset DOI: [10.5061/dryad.9w0vt4bwm](https://doi.org/10.5061/dryad.9w0vt4bwm) ## Description of the data and file structure This file contains the raw data used to generate the figures published in the preprint: Mechanistic Dissection of BLTP2 targeting to ER-PM contact sites. Sarah D. Neuman, Amy T. Cavanagh, Nicole Sheffels, Elizabeth Conibear, Arash Bashirullah This manuscript used *Drosophila* as a model system to investigate the mechanisms that regulate targeting of the bridge-like lipid transfer protein *BLTP2* (*hobbit* in *Drosophila*) to endoplasmic reticulum-plasma membrane (ER-PM) contact sites. This dataset includes quantification of Hobbit cortical puncta, quantification of co-localization by Pearson’s Correlation Coefficient, phylogenetic analysis, absolute quantification of mRNA transcript levels, lethal phase data, body size data, and mucin granule area data. Each file name contains a ..., ,