Oxidative folding yield of DkTx under different conditionsa.
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aEach folding condition contains 1 mM EDTA, 2.5 mM GSH and 0.25 mM GSSH. The concentration of DkTx in the folding solution was 12.5 µM.bA: 0.4 M Tris-HCl (pH 8.0), B: 1 M NH4OAc (pH 8.0).cFolding yield was calculated by comparing the areas of the HPLC peaks for the linear and folded toxin (peak area of correctly folded toxin × 100/peak area of linear toxin).
创建时间:
2015-12-02



