Conserved genes modify <i>Cesmn-1(lf)</i> defects in the growth assay.

C. elegans modifier genes are listed in column 1 and orthologous Drosophila genes are listed in column 2. Only genes that significantly altered the percentage of large Cesmn-1(lf) homozygous animals (column 3) or +/Cesmn-1(lf) heterozygous animals (column 4) are presented. Animals were reared for two generations on bacterial RNAi strains targeting the C. elegans gene prior to growth analysis as described in Figure 1. The percentage of large animals was determined for each genotype after RNAi knockdown of the indicated C. elegans gene and is reported with standard error of the mean (S.E.M.). Large animals include adults, L4 and L3 stage larvae. This assay does not distinguish between slow growth, changes in relative length, and altered viability. Three to six independent determinations were undertaken for each genotype/RNAi culture and the percentage of large animals in each trial was averaged. Significant changes from empty(RNAi) for each RNAi/genotype using the two-tailed Mann-Whitney U test (p<0.05) are indicated with an asterisk. See Table S1 for results for all genes tested and Materials and Methods for details.