Detailed amplification conditions.
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All concentrations are given per reaction. n.p. qPCR was not performed. a5-virus-mix consists of the HIV-1 strains JR-CSF, NL4-3, HXB2, YU2 and 89.6 (see also Materials and Methods). b3/23 µl of cDNA was used for the 1st PCR reaction, this corresponds to ∼4,200 cDNA copies. c10/50 µl of purified, undiluted 1st PCR product was transferred to the 2nd PCR.
创建时间：
2013-09-18
